Name	Type	Attributes	Definition
template_class	string	required	"The class of nucleic acid that was used as primary starting material for the following procedures
"
template_quality	string	required, nullable	Description and results of the quality control performed on the template material
template_amount	number	required, nullable	Amount of template that went into the process
template_amount_unit	:ref:`Ontology <OntoVoc>`	required, nullable	Unit of template amount
library_generation_method	string	required	Generic type of library generation
library_generation_protocol	string	required, nullable	Description of processes applied to substrate to obtain a library that is ready for sequencing
library_generation_kit_version	string	required, nullable	When using a library generation protocol from a commercial provider, provide the protocol version number
pcr_target	array of :ref:`PCRTarget <PCRTargetFields>`	optional	"If a PCR step was performed that specifically targets the IG/TR loci, the target and primer locations need to be provided here. This field holds an array of PCRTarget objects, so that multiplex PCR setups amplifying multiple loci at the same time can be annotated using one record per locus. PCR setups not targeting any specific locus must not annotate this field but select the appropriate library_generation_method instead.
"
complete_sequences	string	required	"To be considered `complete`, the procedure used for library construction MUST generate sequences that 1) include the first V gene codon that encodes the mature polypeptide chain (i.e. after the leader sequence) and 2) include the last complete codon of the J gene (i.e. 1 bp 5' of the J->C splice site) and 3) provide sequence information for all positions between 1) and 2). To be considered `complete & untemplated`, the sections of the sequences defined in points 1) to 3) of the previous sentence MUST be untemplated, i.e. MUST NOT overlap with the primers used in library preparation. `mixed` should only be used if the procedure used for library construction will likely produce multiple categories of sequences in the given experiment. It SHOULD NOT be used as a replacement of a NULL value.
"
physical_linkage	string	required	"In case an experimental setup is used that physically links nucleic acids derived from distinct `Rearrangements` before library preparation, this field describes the mode of that linkage. All `hetero_*` terms indicate that in case of paired-read sequencing, the two reads should be expected to map to distinct IG/TR loci. `*_head-head` refers to techniques that link the 5' ends of transcripts in a single-cell context. `*_tail-head` refers to techniques that link the 3' end of one transcript to the 5' end of another one in a single-cell context. This term does not provide any information whether a continuous reading-frame between the two is generated. `*_prelinked` refers to constructs in which the linkage was already present on the DNA level (e.g. scFv).
"